In vivo intracellular signaling as a marker of antiangiogenic activity.
Cancer Res
; 61(19): 7048-51, 2001 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-11585733
ABSTRACT
Alterations in endothelial cell (EC) signaling could serve as a marker of effective antiangiogenic therapy. We determined the effect of an antiangiogenic tyrosine kinase inhibitor, SU6668, on tumor EC signaling in liver metastases in mice. In vitro immunofluorescence verified that pretreatment of ECs with SU6668 before exposure to VEGF decreased in vitro phosphorylation of Erk and Akt. Using double-fluorescence immunohistochemistry, phosphorylated Erk and Akt were constitutively expressed in ECs in liver metastases in untreated mice, but SU6668 blocked activation of these signaling intermediates. Determining the activation status of the Erk and Akt signaling pathways in tumor ECs may serve as a surrogate marker for the effectiveness of antiangiogenic regimens.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pirroles
/
Proteínas Serina-Treonina Quinasas
/
Inhibidores de la Angiogénesis
/
Sistema de Señalización de MAP Quinasas
/
Indoles
/
Neoplasias Hepáticas
/
Neovascularización Patológica
Idioma:
En
Revista:
Cancer Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos