Random mutagenesis of human cytochrome p450 2A6 and screening with indole oxidation products.
Arch Biochem Biophys
; 395(1): 25-31, 2001 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-11673862
ABSTRACT
Cytochrome P450 (P450) 2A6 mutants from randomized libraries generated in the substrate recognition sequence (SRS) regions were screened in Escherichia coli on the basis of indole metabolism. SRS 3 and 4 libraries yielded colonies that produced indigo at least as well as wild-type (WT) P450 2A6, and some colonies were consistently more blue upon replating. One mutant, F209T, showed indole 3-hydroxylation
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Hidrocarburo de Aril Hidroxilasas
/
Mutagénesis
/
Sistema Enzimático del Citocromo P-450
/
Oxigenasas de Función Mixta
/
Indoles
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
En
Revista:
Arch Biochem Biophys
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos