Protein-lipid interactions in reconstituted high density lipoproteins: apolipoprotein and cholesterol influence.

ABSTRACT

Two fluorescent probes-cis- and trans-parinaric acids were used to study the dimensions, lipid dynamics and apolipoprotein location in the reconstituted discoidal high density lipoproteins (rHDL). The rHDL particles made from apolipoprotein A-I (apoA-I), dipalmitoylphosphatidylcholine (DPPC), with or without cholesterol (Chol) were compared with the analogous particles with two other apolipoproteins-apoE and apoA-II. The data obtained for apoA-I-containing rHDL were as follows (1) the inclusion of 8 mol.% of cholesterol did not significantly change the particle dimensions (13+/-1 nm) or the mean distance between apoA-I and the disc axis; (2) the phospholipid domains-boundary lipid region in the close vicinity to apoA-I molecule and the remaining part of the bilayer-existed at temperatures both lower and above DPPC transition temperature T(t); (3) at Tradius of 2.8 nm that conserved partially after DPPC phase transition; (4) inhomogeneous cholesterol distribution was assumed to exist within these domains. A hydrophobic matching concept was used to compare protein-lipid interactions in rHDL particles. For complexes with all three apolipoproteins studied, at Tlipid phase of rHDL was significantly higher compared to pure DPPC bilayer. After temperature-induced transition, mobility increased significantly still being lower in rHDL. The comparative study of lipid dynamics in apoA-I-, apoE- and apoA-II-containing complexes revealed the presence of boundary lipid in all three complexes without cholesterol. The degree of cholesterol exclusion from the boundary lipid region seems to increase in the order A-Iapolipoprotein molecule.