Immobilized-metal-ion affinity chromatography as a tool for the qualitative study of pepsinogen phosphorylation.
J Biochem Biophys Methods
; 49(1-3): 523-31, 2001 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-11694299
ABSTRACT
Affinity chromatography on immobilized Fe(3+) ions--immobilized-metal-ion affinity chromatography (IMAC) method--was used for the determination of pepsin and pepsinogen phosphorylation. IMAC is a very powerful method for detailed studies of proteins. Dephosphorylation of the pepsinogens and pepsins has no effect on their proteolytic ability. For this reason, the determination of proteolytic activity was used for the detection of pepsinogen (pepsin) presence in the collected fractions as a very suitable and specific method. Pepsins and their zymogens probably have the same amounts of phosphate ions in their molecule. The exact definition of conditions is very important for the prepurification of the proteinases and for their analysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pepsinógeno A
/
Mucosa Gástrica
Tipo de estudio:
Qualitative_research
Límite:
Humans
Idioma:
En
Revista:
J Biochem Biophys Methods
Año:
2001
Tipo del documento:
Article
País de afiliación:
República Checa