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Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples.
Fang, Ying; Wu, Wai-Hong; Pepper, Jessica L; Larsen, Jill L; Marras, Salvatore A E; Nelson, Eric A; Epperson, William B; Christopher-Hennings, Jane.
Afiliación
  • Fang Y; Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA.
J Clin Microbiol ; 40(1): 287-91, 2002 Jan.
Article en En | MEDLINE | ID: mdl-11773135
An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimum quantity of 1.7 x 10(-4) pg of DNA, correlating to 1 to 8 CFU, was detected.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paratuberculosis / Enfermedades de los Bovinos / Reacción en Cadena de la Polimerasa / Mycobacterium avium subsp. paratuberculosis / Heces Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals Idioma: En Revista: J Clin Microbiol Año: 2002 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paratuberculosis / Enfermedades de los Bovinos / Reacción en Cadena de la Polimerasa / Mycobacterium avium subsp. paratuberculosis / Heces Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals Idioma: En Revista: J Clin Microbiol Año: 2002 Tipo del documento: Article País de afiliación: Estados Unidos
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