Quantal release of free radicals during exocytosis of phagosomes.
Nat Cell Biol
; 4(4): 279-85, 2002 Apr.
Article
en En
| MEDLINE
| ID: mdl-11901421
ABSTRACT
Secretion of lysosomes and related organelles is important for immune system function. High-resolution membrane capacitance techniques were used to track changes in membrane area in single phagocytes during opsonized polystyrene bead uptake and release. Secretagogue stimulation of cells preloaded with beads resulted in immediate vesicle discharge, visualized as step increases in capacitance. The size of the increases were consistent with phagosome size. This hypothesis was confirmed by direct observation of dye release from bead-containing phagosomes after secretagogue stimulation. Capacitance recordings of exocytosis were correlated with quantal free radical release, as determined by amperometry. Thus, phagosomes undergo regulated secretion in macrophages, one function of which may be to deliver sequestered free radicals to the extracellular space.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fagosomas
/
Proteínas de Transporte Vesicular
/
Radicales Libres
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Cell Biol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos