Efficiency of lipofection of adherent cells is limited by apoptosis.
Folia Histochem Cytobiol
; 40(2): 133-4, 2002.
Article
en En
| MEDLINE
| ID: mdl-12056611
ABSTRACT
Stability of gene expression and transfection efficiency plays the main role in the application of gene transfer method. In somatic cell gene delivery, expression of the gene product is limited by the function of the cell to which it is delivered. In the present study analyzing the lipofected adherent cells, we have shown that lower level of transgene beta-galactosidase activity at later time period correlated with decrease in cell viability, which was shown to be due to apoptosis. Apoptosis following DNA uptake occurred only when DNA was present during lipofection.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Transfección
/
Adhesión Celular
/
Apoptosis
Límite:
Humans
Idioma:
En
Revista:
Folia Histochem Cytobiol
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Polonia