Determination of a chemopreventive agent, Oltipraz, in rat plasma and urine by high-performance liquid chromatography.
Res Commun Mol Pathol Pharmacol
; 110(1-2): 133-8, 2001.
Article
en En
| MEDLINE
| ID: mdl-12090351
ABSTRACT
A high-performance liquid chromatographic method was developed for the determination of a chemopreventive agent, Oltipraz, in rat plasma and urine. The sample preparation was simple; 2 volumes of acetonitrile were added to deproteinize the biological sample. A 50-microl aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase, acetonitrile 0.5 mM ammonium acetate (55 45, v/v for rat plasma and 45 55, v/v for rat urine), was run at a flow-rate of 1.5 ml/min. The column effluent was monitored using an ultraviolet detector set at 305 nm. The retention times for Oltipraz in rat plasma and urine were approximately 5.8 and 8.6 min, respectively. The detection limits of Oltipraz in rat plasma and urine were 20 and 50 ng/ml, respectively. The coefficients of variation of the assay (within-day and between-day) were generally low (below 4.65%) in concentration ranges from 0.02 (0.05) to 10 microg/ml for rat plasma and urine. No interference from endogenous substances was found.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_zoonosis
Asunto principal:
Pirazinas
/
Esquistosomicidas
Límite:
Animals
Idioma:
En
Revista:
Res Commun Mol Pathol Pharmacol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
FARMACOLOGIA
/
PATOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Corea del Sur