Rapid parallel mutation scanning of gene fragments using a microelectronic protein-DNA chip format.
Nucleic Acids Res
; 30(14): e64, 2002 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-12136112
ABSTRACT
We have developed a method for the de novo discovery of genetic variations, including single nucleotide polymorphisms and mutations, on microelectronic chip devices. The method combines the features of electronically controlled DNA hybridisation on open-format microarrays, with mutation detection by a fluorescence-labelled mismatch- binding protein. Electronic addressing of DNA strands to distinct test sites of the chip allows parallel analysis of several individuals, as demonstrated for mutations in different exons of the p53 gene. This microelectronic chip-based mutation discovery assay may substitute for time-consuming sequencing studies and will complement existing technologies in genomic research.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Análisis Mutacional de ADN
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Proteínas de Unión al ADN
Límite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2002
Tipo del documento:
Article
País de afiliación:
Alemania