[Modification of HPV type 16 E6 and E7 genes, and analysis of stability and immunogenicity of the modified proteins].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 16(2): 124-7, 2002 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-12196821
ABSTRACT
BACKGROUND:
To select the mutants of HPV type 16 E6 and E7 genes suitable for construction of vaccine for treatment of cervical cancer.METHODS:
E6 and E7 genes were modified by site-directed mutagenesis. Several recombinant vaccina viruses were constructed by inserting the E6 or E7 mutants into the genome of vaccina virus Tiantan strain and employed to study their antigenicity.RESULTS:
Western blot assay showed that the E6 ?mutant? with substitution of Gly for Leu at amino acid site 50 and E7 mutant with substitution of Gly for Cys-24 and Glu-26 had no effect on their stability and antigenicity, but change of the Cys at position 91 of E7 dramatically reduced its stability and antigencity. Conclusion The results confirmed that the Zinc-finger structure at the E7 C-terminal? plays an important role in the integrity and stability of E7 protein.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
4_TD
Problema de salud:
4_smallpox
Asunto principal:
Papillomaviridae
/
Proteínas Represoras
/
Virus Vaccinia
/
Proteínas Oncogénicas Virales
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Asunto de la revista:
VIROLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
China