Antitumor effect to IL-12 administration into the portal vein on murine liver metastasis.
J Hepatobiliary Pancreat Surg
; 9(4): 503-10, 2002.
Article
en En
| MEDLINE
| ID: mdl-12483274
ABSTRACT
BACKGROUND/PURPOSE:
Interleukin (IL)-12 has been shown to possess potent antitumor activity, and an antitumor effect of systemic IL-12 administration has been reported in liver metastasis models.METHODS:
In this study, we examined the usefulness of local IL-12 administration into the portal system in the treatment of liver metastasis. First, we confirmed the antitumor effect of recombinant IL-12 (rIL-12) on MC-38 tumors in an intracutaneous model. In the murine liver metastasis model, 1 x 10(5) of MC-38 cells were injected into the portal vein on day 0, and the spleen was transpositioned subcutaneously for administration of rIL-12 continually into the portal system. From days 3 to 7, 0.1 micro g rIL-12 was administered intraperitoneally or intrasplenicly, while Hanks' Balanced Salf Solution (HBSS) was injected intrasplenicly in the control group.RESULTS:
The liver weight in the rIL-12 intraperitoneal treatment group (1.88 +/- 0.37 g) and that in the rIL-12 intrasplenic treatment group (1.43 +/- 0.21 g) were significantly less than that in the HBSS group (2.86 +/- 0.74 g; P < 0.05). The numbers of metastatic nodules in the rIL-12 intraperitoneal treatment group (22.3 +/- 17.1) and in the rIL-12 intrasplenic treatment group (12.4 +/- 13.8) were significantly less than that in the HBSS group (137.1 +/- 44.9; P < 0.05). Complete regression of the tumor was observed in one of six mice in the rIL-12 intrasplenic treatment group. This antitumor effect of rIL-12 on MC-38 liver metastasis was not observed in interferon (IFN)-gamma knockout mice. Intraportal administration of IL-12-transduced fibroblasts, which were syngeneic to C57BL/6 mice, had an antitumor effect in the MC-38 liver metastasis model.CONCLUSIONS:
These results suggested that the local administration of IL-12 into the portal system would be a useful strategy for the treatment of liver metastasis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vena Porta
/
Adenocarcinoma
/
Adyuvantes Inmunológicos
/
Neoplasias del Colon
/
Interleucina-12
/
Neoplasias Hepáticas Experimentales
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Hepatobiliary Pancreat Surg
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Japón