Myosin heavy chain composition in human masticatory muscles by immunohistochemistry and gel electrophoresis.
J Histochem Cytochem
; 51(1): 113-9, 2003 Jan.
Article
en En
| MEDLINE
| ID: mdl-12502760
ABSTRACT
In this study we compared the immunohistochemically quantified fiber type area with the myosin heavy chain (MyHC) contents of a bundle of fibers from a human masticatory muscle. The total cross-sectional areas were determined immunohistochemically for the three major fiber types (I, IIA, and IIX) in bundles of fibers (n=42) taken from the anterior and posterior belly of the human digastric muscle (n=7). The relative MyHC contents of the same fiber bundles were determined electrophoretically (MyHC-I, -IIA, and -IIX; anterior, 32%, 35%, and 33%; posterior, 39%, 42%, and 19%) and compared with the immunohistochemical data (MyHC-I, -IIA, and -IIX; anterior, 32%, 31%, and 37%; posterior, 39%, 45%, and 15%). No significant differences were seen in the mean fiber type distribution between the two techniques; the correlation coefficient ranged from 0.71 to 0.96. The correlation coefficient was higher for MyHC type I and MyHC type IIX than for MyHC type IIA. The MyHC contents of single fibers taken from the posterior belly indicated that many fibers in this belly co-express MyHC-IIA and MyHC-IIX. Despite the presence of these hybrid fibers, the correspondence between both methods was relatively large.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cadenas Pesadas de Miosina
/
Músculos Masticadores
Límite:
Aged
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Histochem Cytochem
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Países Bajos