Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping.
Cell Res
; 12(5-6): 401-5, 2002 Dec.
Article
en En
| MEDLINE
| ID: mdl-12528898
ABSTRACT
Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromosome 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed. Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatocitos
/
Mapeo Cromosómico
/
Cromosomas
/
Técnicas de Cultivo de Célula
/
Sus scrofa
/
Meiosis
Límite:
Animals
Idioma:
En
Revista:
Cell Res
Año:
2002
Tipo del documento:
Article
País de afiliación:
China