Internal initiation drives the synthesis of Ure2 protein lacking the prion domain and affects [URE3] propagation in yeast cells.
EMBO J
; 22(5): 1199-209, 2003 Mar 03.
Article
en En
| MEDLINE
| ID: mdl-12606584
ABSTRACT
The [URE3] phenotype in Saccharomyces cerevisiae is caused by the inactive, altered (prion) form of the Ure2 protein (Ure2p), a regulator of nitrogen catabolism. Ure2p has two functional domains an N-terminal domain necessary and sufficient for prion propagation and a C-terminal domain responsible for nitrogen regulation. We show here that the mRNA encoding Ure2p possesses an IRES (internal ribosome entry site). Internal initiation leads to the synthesis of an N-terminally truncated active form of the protein (amino acids 94-354) lacking the prion-forming domain. Expression of the truncated Ure2p form (94-354) mediated by the IRES element cures yeast cells of the [URE3] phenotype. We assume that the balance between the full-length and truncated (94-354) Ure2p forms plays an important role in yeast cell physiology and differentiation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Iniciación de la Cadena Peptídica Traduccional
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Saccharomyces cerevisiae
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Biosíntesis de Proteínas
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Priones
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Proteínas de Saccharomyces cerevisiae
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
2003
Tipo del documento:
Article
País de afiliación:
Suiza