Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography.
Electrophoresis
; 24(6): 1031-7, 2003 Mar.
Article
en En
| MEDLINE
| ID: mdl-12658692
ABSTRACT
The separation and on-line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on-line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were applied; the optimum on-line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; approximately 1/10 to the value of LD(50)). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Cromatografía Líquida de Alta Presión
/
Cromatografía Capilar Electrocinética Micelar
/
Dietilamida del Ácido Lisérgico
Límite:
Animals
Idioma:
En
Revista:
Electrophoresis
Año:
2003
Tipo del documento:
Article
País de afiliación:
Taiwán