Determination of antibacterial flomoxef in serum by capillary electrophoresis.
J Chromatogr Sci
; 41(4): 173-6, 2003 Apr.
Article
en En
| MEDLINE
| ID: mdl-12803802
ABSTRACT
A determination method of flomoxef (FMOX) concentration in serum by capillary electrophoresis is developed. Serum samples are extracted with acetonitrile. After pretreatment, they are separated in a fused-silica capillary tube with a 25 mM borate buffer (pH 10.0) as a running buffer that contains 50mM sodium dodecyl sulfate. The FMOX and acetaminophen (internal standard) are detected by UV absorbance at 200 nm. Linearity (0-200 mg/L) is good, and the minimum limit of detection is 1.0 mg/L (S/N = 3). The relative standard deviations of intra- and interassay variability are 1.60-4.78% and 2.10-3.31%, respectively, and the recovery rate is 84-98%. This method can be used for determination of FMOX concentration in serum.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cefalosporinas
/
Electroforesis Capilar
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr Sci
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón