[Primary nasal epithelial cells and fibroblasts have inflammation-inducing functions]. / Primäre nasale Epithelzellen und Fibroblasten haben entzündungsfördernde Eigenschaften.
HNO
; 51(6): 480-5, 2003 Jun.
Article
en De
| MEDLINE
| ID: mdl-12835847
ABSTRACT
BACKGROUND:
The appearance of neutrophils in rhinitis and sinusitis led to the working hypothesis that neutrophil-specific attractants commonly called chemokines are generated by stimulation with proinflammatory cytokines and bacteria. The receptor mechanism of chemokine synthesis by bacterial products is under discussion and still has to be elucidated. PATIENTS ANDMETHODS:
The primary nasal cultures of epithelial cells and fibroblasts ( n=4) were incubated with TNF-alpha (tumor necrosis factor) for 24 and 72 h. Bacterial stimulation of the cell cultures was performed by adding supernatants from the mucoid phenotype of Pseudomonas aeruginosa (PA01) in dilution 15 for 24 and 72 h. Supernatants were collected and the concentration of the chemokines interleukin-8 (IL-8), GRO-alpha (growth-related oncogene-alpha), and ENA-78 (epithelial neutrophil-activating peptide) were determined by ELISA technique. Our results revealed that the protein concentration of the chemokines GRO-alpha and IL-8 was upregulated by TNF-alpha as well as by bacterial supernatants in epithelial cells.CONCLUSION:
We conclude that GRO-alpha and IL-8 were inducible by bacterial supernatants in nasal epithelial cells and fibroblasts.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
Problema de salud:
2_enfermedades_transmissibles
Asunto principal:
Pseudomonas aeruginosa
/
Interleucina-8
/
Quimiocinas
/
Células Epiteliales
/
Fibroblastos
/
Mucosa Nasal
Límite:
Humans
Idioma:
De
Revista:
HNO
Año:
2003
Tipo del documento:
Article