[An experimental study of inhibiting effect of decoy oligodeoxynucleotides on the gene expression of collagen in NIH3T3 cell].
Zhonghua Shao Shang Za Zhi
; 19(3): 175-8, 2003 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-12921625
ABSTRACT
OBJECTIVE:
To investigate the effect of activator protein-1 (AP-1) decoy-oligodeoxynucleotides (Decoy-ODNs) on the expression of fibroblast alpha2 type I collagen, so as to explore the gene therapy of pathologic scar.METHODS:
Decoy-ODNs targeting AP-1 were designed and synthesized. NIH3T3 cells were transfected by cationic liposomes. The distribution of Decoy-ODNs in the cells was investigated. The inhibiting effects of Decoy-ODNs on AP-1 were determined by electrophoretic mobility shift assay (EMSA). And the effects of Decoy-ODNs on the collagen synthesis in the cells were analyzed by RT-PCR.RESULTS:
AP-1 Decoy-ODNs could competitively inhibit the AP-1 in vitro activity. Cationic liposomes could play roles by effectively transfecting Decoy-ODNs into the plasma and nucleus. The mRNA expression of fibroblast alpha2 type I collagen decreased evidently after 24 hours of Decoy-ODNs action.CONCLUSION:
Decoy-ODNs could inhibit the mRNA expression of fibroblast alpha2 type I collagen by antagonizing AP-1.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
/
Factor de Transcripción AP-1
/
Colágeno Tipo I
/
Fibroblastos
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Shao Shang Za Zhi
Asunto de la revista:
TRAUMATOLOGIA
Año:
2003
Tipo del documento:
Article