Hepatitis C virus-like particle budding: role of the core protein and importance of its Asp111.
J Virol
; 77(18): 10131-8, 2003 Sep.
Article
en En
| MEDLINE
| ID: mdl-12941925
ABSTRACT
In the absence of a hepatitis C virus (HCV) culture system, the use of a Semliki Forest virus replicon expressing genes encoding HCV structural proteins that assemble into HCV-like particles provides an opportunity to study HCV morphogenesis. Using this system, we showed that the HCV core protein constitutes the budding apparatus of the virus and that its targeting to the endoplasmic reticulum by means of the signal sequence of E1 protein is essential for budding. In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virión
/
Proteínas del Núcleo Viral
/
Hepacivirus
Límite:
Animals
Idioma:
En
Revista:
J Virol
Año:
2003
Tipo del documento:
Article
País de afiliación:
Francia