Measurement of plasma hydroperoxide concentration by FOX-1 assay in conjunction with triphenylphosphine.
Clin Chim Acta
; 337(1-2): 147-52, 2003 Nov.
Article
en En
| MEDLINE
| ID: mdl-14568191
ABSTRACT
INTRODUCTION:
Hydroperoxides are well-recognized reactive oxygen species which are associated with oxidative stress, a phenomenon of current clinical interest as oxidative stress is associated with a number of disease condition. Ferrous ion oxidation xylenol orange (FOX) methods of hydroperoxide estimation has outdated other methods available for hydroperoxide estimation. Two versions FOX assays are described in the literature, FOX-1 and FOX-2, in which FOX-1 is more sensitive.METHODS:
we increased the sensitivity of FOX-1 assay by stabilizing the reagent pH 1.7-1.8. Analogous to FOX-2 assay, we have modified FOX-1 assay by using it in conjunction with triphenylphosphine and butylated hydroxytoluene, thus increasing the specificity of FOX-1 assay for hydroperoxide. By modified FOX-1 method, we estimated plasma hydroperoxide concentration of normal human subjects and of diabetic patients and compared with FOX-2 method.RESULTS:
The FOX-1 method showed a significant high value of plasma hydroperoxide concentration compared to FOX-2 method both in normal subjects and diabetic patients with a significant correlation. By modified FOX-1 method, the recovery percentage of cumene hydroperoxide was better in biological samples when compared to FOX-2 method.CONCLUSION:
The modified FOX-1 method is equally specific for hydroperoxide determination when compared to FOX-2 but is more sensitive.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organofosforados
/
Xilenos
/
Compuestos Férricos
/
Compuestos Ferrosos
/
Compuestos de Amonio Cuaternario
/
Peróxido de Hidrógeno
Límite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Año:
2003
Tipo del documento:
Article
País de afiliación:
India