Discrete types of osteoclast precursors can be generated from embryonic stem cells.
Stem Cells
; 21(6): 670-80, 2003.
Article
en En
| MEDLINE
| ID: mdl-14595127
ABSTRACT
Osteoclast precursors (OCPs) share some characteristics with the monocyte/macrophage lineages, but the early events of OCP development are not yet clear. To investigate osteoclastogenesis from the earliest stage, we used step-wise cultures of embryonic stem (ES) cells to induce mature osteoclasts and assessed the effect of vascular endothelial growth factor receptor (VEGFR)-1/Fc chimeric protein on osteoclast development. Addition of VEGFR-1/Fc for the first 5 days of culture (phase I) severely inhibited the development of OCPs. Although OCPs were detected after culturing for a further 5 days (phase II), the reduction of OCPs in phase I was maintained in phase II. The generation of OCPs in phase I was resistant to signal blocking mediated by Kit receptors, but that in phase II was partially inhibited by either an anti-Kit antagonistic antibody or VEGFR-1/Fc and was severely inhibited by the combination of both reagents. Moreover, the OCPs in phase I gave rise to larger numbers of osteoclasts but required a longer period for maturation than the OCPs in phase II. We thus showed that OCPs expanded in phase II, but the majority of OCPs arose from ES cells in a manner dependent on VEGFR-1 binding factor(s) in phase I.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoclastos
/
Células Madre
/
Linaje de la Célula
/
Embrión de Mamíferos
Límite:
Animals
Idioma:
En
Revista:
Stem Cells
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón