[Investigation of p53 gene mutations in keloids using PCR-SSCP].

ABSTRACT

OBJECTIVE: To detect gene mutations of p53 gene (exon 4-6) in fibroblasts. METHODS: Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing. RESULTS: Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids. CONCLUSION: Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.