Deep hypothermia during ischemia improves functional recovery and reduces free-radical generation in isolated reperfused rat heart.
J Heart Lung Transplant
; 23(4): 487-91, 2004 Apr.
Article
en En
| MEDLINE
| ID: mdl-15063410
ABSTRACT
BACKGROUND:
We investigated the influence of deep hypothermia (4 degrees C) during ischemia-reperfusion in the isolated rat heart model.METHODS:
Isolated, perfused rat hearts underwent either 30 minutes of normothermic ischemia (control group) or 30 minutes of hypothermic ischemia (hypothermia-treated group), followed by 30 minutes of reperfusion in both groups. We recorded functional parameters and used electron spin resonance (ESR) spectroscopy to detect ascorbyl radicals, as markers of free-radical production, in samples of coronary effluents.RESULTS:
Functional parameters were stable in the 2 groups during pre-ischemic and ischemic periods. During reperfusion, coronary flow, left diastolic ventricular pressure, left ventricular developed pressure, and heart rate more rapidly recovered to values close to those obtained during the pre-ischemic period in the hypothermia-treated group than in the control group. Moreover, the post-ischemic contracture observed in the control group did not appear in the hypothermia-treated group. Finally, ESR analysis showed that the post-ischemic release of ascorbyl radicals decreased in the hypothermia-treated group.CONCLUSIONS:
These results demonstrate that the protective effect of hypothermia against functional injury caused by ischemia-reperfusion may decrease the free-radical burst at reperfusion.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Daño por Reperfusión
/
Isquemia Miocárdica
/
Circulación Coronaria
/
Ácido Deshidroascórbico
/
Hipotermia Inducida
Límite:
Animals
Idioma:
En
Revista:
J Heart Lung Transplant
Asunto de la revista:
CARDIOLOGIA
/
TRANSPLANTE
Año:
2004
Tipo del documento:
Article
País de afiliación:
Francia