Single-cell detection of trans-splicing ribozyme in vivo activity.
J Am Chem Soc
; 126(23): 7158-9, 2004 Jun 16.
Article
en En
| MEDLINE
| ID: mdl-15186136
The Tetrahymena trans-splicing ribozyme can edit RNA in a sequence-specific manner, but its efficiency needs to be improved for any functional rescues. This communication describes a simple method that uses a bacterial enzyme beta-lactamase to report trans-splicing activity of Tetrahymena ribozyme in single living mammalian cells by fluorescence microscopy and flow cytometry. This enzyme-based single-cell detection method is highly sensitive and compatible with living cell flow cytometry, and should allow a cell-based systematic screening of a vast library of ribozymes for better trans-spliced ribozyme variants.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Catalítico
/
Trans-Empalme
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Am Chem Soc
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos