[Effects of baicalin on the expression of pro-MMP-1 and MMP-3 in human gingival fibroblasts and periodontal ligament cells].
Zhonghua Kou Qiang Yi Xue Za Zhi
; 39(3): 197-200, 2004 May.
Article
en Zh
| MEDLINE
| ID: mdl-15196384
ABSTRACT
OBJECTIVE:
To investigate the influence of baicalin on the IL-1beta induced pro-MMP-1 in HGF and the effects of baicalin on MMP-3 expression in periodontal ligament cells (PDLCs).METHODS:
The amount of secreted pro-MMP-1 and MMP-3 expression was detected by ELISA and cell immunochemistry.RESULTS:
(1) The amount of secreted pro-MMP-1 (3.333 +/- 0.123) microg/L increased significantly following 1 microg/L of IL-1beta, compared with control group (1.960 +/- 0.180) microg/L. Addition of baicalin to cell culture medium for 1 hour following IL-1beta decreased pro-MMP-1 secretion in a dose-dependent manner in the range of 10 approximately 1,000 microg/L. (2) 1 microg/L IL-1beta could significantly stimulate the synthesis and secretion of MMP-3 in PDLCs. (3) The baicalin could not interfere the synthesis of MMP-3, but could inhibit the release of MMP-3 from PDLCs.CONCLUSIONS:
Baicalin could inhibit the secretion of pro-MMP-1 and MMP-3 expression in IL-1beta induced HGF and PDLCs, which suggests that baicalin may play an important role in preventing and treating periodontal disease.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ligamento Periodontal
/
Flavonoides
/
Metaloendopeptidasas
/
Colagenasas
/
Precursores Enzimáticos
/
Encía
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Kou Qiang Yi Xue Za Zhi
Asunto de la revista:
ODONTOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China