Downregulation of peroxiredoxin V stimulates formation of etoposide-induced double-strand DNA breaks.
FEBS Lett
; 572(1-3): 75-9, 2004 Aug 13.
Article
en En
| MEDLINE
| ID: mdl-15304327
ABSTRACT
Antioxidant protein Peroxiredoxin V (PrxV) is located in mitochondria and peroxisomes but is also present in the nucleus. Here, we show that nuclear PrxV associates with coilin-containing bodies suggesting possible interaction of this protein with transcription complexes. We also studied etoposide-induced phosphorylation of histone H2AX (gamma-H2AX) in human cells in which PrxV activity was downregulated (knockdown, KD-clones) or compromised by overexpression of redox-negative (RD) protein. In KD clones, but not in RD-clones, formation of etoposide-induced gamma-H2AX was increased, indicating that PrxV inhibits conversion of topoisomerase II cleavage complexes into double-strand DNA breaks but this inhibition is not caused by its antioxidant activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peroxidasas
/
Daño del ADN
/
Regulación de la Expresión Génica
/
Etopósido
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2004
Tipo del documento:
Article
País de afiliación:
Rusia