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Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower.
Kolkman, Judith M; Slabaugh, Mary B; Bruniard, Jose M; Berry, Simon; Bushman, B Shaun; Olungu, Christine; Maes, Nele; Abratti, Gustavo; Zambelli, Andres; Miller, Jerry F; Leon, Alberto; Knapp, Steven J.
Afiliación
  • Kolkman JM; Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA.
Theor Appl Genet ; 109(6): 1147-59, 2004 Oct.
Article en En | MEDLINE | ID: mdl-15309298
ABSTRACT
Wild biotypes of cultivated sunflower ( Helianthus annuus L.) are weeds in corn ( Zea mays L.), soybean ( Glycine max L.), and other crops in North America, and are commonly controlled by applying acetohydroxyacid synthase (AHAS)-inhibiting herbicides. Biotypes resistant to two classes of AHAS-inhibiting herbicides-imidazolinones (IMIs) or sulfonylureas (SUs)-have been discovered in wild sunflower populations (ANN-PUR and ANN-KAN) treated with imazethapyr or chlorsulfuron, respectively. The goals of the present study were to isolate AHAS genes from sunflower, identify mutations in AHAS genes conferring herbicide resistance in ANN-PUR and ANN-KAN, and develop tools for marker-assisted selection (MAS) of herbicide resistance genes in sunflower. Three AHAS genes ( AHAS1, AHAS2, and AHAS3) were identified, cloned, and sequenced from herbicide-resistant (mutant) and -susceptible (wild type) genotypes. We identified 48 single-nucleotide polymorphisms (SNPs) in AHAS1, a single six-base pair insertion-deletion in AHAS2, and a single SNP in AHAS3. No DNA polymorphisms were found in AHAS2 among elite inbred lines. AHAS1 from imazethapyr-resistant inbreds harbored a C-to-T mutation in codon 205 ( Arabidopsis thaliana codon nomenclature), conferring resistance to IMI herbicides, whereas AHAS1 from chlorsulfuron-resistant inbreds harbored a C-to-T mutation in codon 197, conferring resistance to SU herbicides. SNP and single-strand conformational polymorphism markers for AHAS1, AHAS2, and AHAS3 were developed and genetically mapped. AHAS1, AHAS2, and AHAS3 mapped to linkage groups 2 ( AHAS3), 6 ( AHAS2), and 9 ( AHAS1). The C/T SNP in codon 205 of AHAS1 cosegregated with a partially dominant gene for resistance to IMI herbicides in two mutant x wild-type populations. The molecular breeding tools described herein create the basis for rapidly identifying new mutations in AHAS and performing MAS for herbicide resistance genes in sunflower.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetolactato Sintasa / Compuestos de Sulfonilurea / Imidazolinas / Helianthus / Herbicidas / Inmunidad Innata / Mutación Idioma: En Revista: Theor Appl Genet Año: 2004 Tipo del documento: Article País de afiliación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetolactato Sintasa / Compuestos de Sulfonilurea / Imidazolinas / Helianthus / Herbicidas / Inmunidad Innata / Mutación Idioma: En Revista: Theor Appl Genet Año: 2004 Tipo del documento: Article País de afiliación: Estados Unidos
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