[Construction and immune potency of recombinant adenovirus containing codon-modified HIV-1 gp120].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 18(2): 113-7, 2004 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-15340495
ABSTRACT
BACKGROUND:
To construct replication-deficient recombinant adenovirus expressing wild and codon-modified HIV-1 gp120.METHODS:
The viral codons were changed to the codon usage of highly expressed mammal gene, the resulting modified gp120 gene was synthesized. The wild and modified gp120 genes were cloned into shuttle vector pShuttle-CMV respectively, and then the constructed plasmids containing gp120 gene was cotransformed with the backbone vector pADeasy-1 into E.coli BJ5183. Transfection of the recombinant AdEasy plasmid into 293 cells was performed to obtain recombinant adenoviruses. The mice were immunized with the recombinant adenoviruses. Their immunogenicity was evaluated by testing antibody and CTL levels of immunized mice.RESULTS:
Two strains of recombinant adenovirus expressing wild and codon-modified HIV-1 gp120 were obtained. The protein expressing level of the recombinant adenoviruses containing modified genes was much higher than that containing wild genes. The mice immunized with recombinant adenoviruses elicited HIV-1 specific antibody and CTL response. The rAd-mod gp120 group was better than the rAd-wt gp120 group.CONCLUSION:
Replication-deficient recombinant adenovirus expressing HIV-1 gp120 can elicit HIV-1 specific humoral and cellular response, the codon-modified recombinant virus was more efficient than the native.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína gp120 de Envoltorio del VIH
/
Adenoviridae
/
VIH-1
/
Vacunas contra el SIDA
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Asunto de la revista:
VIROLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
China