Diurnal variation and age-related changes of bone turnover markers in female Göttingen minipigs.
Lab Anim
; 38(4): 439-46, 2004 Oct.
Article
en En
| MEDLINE
| ID: mdl-15479560
ABSTRACT
We investigated diurnal variation and age-related changes in bone turnover markers in female Gottingen minipigs. Ten females, 6-9 months of age, were used for confirmation of diurnal variation. Blood was collected at 3 h intervals for 24 h, and bone-specific alkaline phosphatase and intact osteocalcin (OC) levels were determined by enzyme immunoassay and radioimmunoassay, respectively. Urine was collected at 3 h intervals for 24 h using a tray attached to the bottom of the cage. The levels of N-terminal telopeptide of type I collagen (NTX) were determined by enzyme immunoassay. Pyridinoline and deoxypyridinoline were measured by high performance liquid chromatography. OC and NTX exhibited diurnal variation (Kruskal-Wallis test, P < 0.05), with the highest and lowest levels at 1800 h (76.7 +/- 26.2 ng/ml) and 0600 h (44.3 +/- 10.3 ng/ml), and at 0300-0559 h (550.4 +/- 82.4 nmol/micromol Cr) and 1200-1459 h (297.8 +/- 152.5 nmol/micromol Cr), respectively. In the study of age-related changes, blood and urine samples from 66 females (age range, 3-76 months) were examined to determine the bone turnover markers. All markers showed high correlations with age (0.569 < R(2) < 0.818). High levels of bone turnover markers were observed in young animals, decreasing with age (Kruskal-Wallis test, P < 0.01). The diurnal variation and age-related changes revealed in the present study will be useful in studies of bone diseases using female Gottingen minipigs.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Porcinos Enanos
/
Huesos
/
Ritmo Circadiano
/
Remodelación Ósea
Límite:
Animals
Idioma:
En
Revista:
Lab Anim
Año:
2004
Tipo del documento:
Article
País de afiliación:
Japón