Crystallization, data collection and phasing of infestin 4, a factor XIIa inhibitor.
Acta Crystallogr D Biol Crystallogr
; 60(Pt 11): 2051-3, 2004 Nov.
Article
en En
| MEDLINE
| ID: mdl-15502324
ABSTRACT
Infestin is a protein from Triatoma infestans (kissing bug) composed of seven Kazal-type domains that is further processed to yield several serine protease inhibitors with varying specificities. Infestins 3 and 4 are the last two domains of the infestin gene and are found in vivo in the insect's anterior midgut. The last domain, infestin 4, has been cloned, expressed and purified. Here, the crystallization of infestin 4 using the sitting-drop vapour-diffusion method with PEG 8000 as precipitant is described. Crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 25.89, b = 45.64, c = 57.41 A. X-ray diffraction data were collected to a maximum resolution of 1.8 A using a synchrotron-radiation source. Initial phases were calculated by molecular replacement using an edited rhodniin molecule as the search model. Structure refinement is in progress.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Triatoma
/
Proteínas Sanguíneas
/
Proteínas de Insectos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Año:
2004
Tipo del documento:
Article
País de afiliación:
Brasil