Effect of magnesium on essential oil formation of genetically transformed and non-transformed chamomile cultures.
J Am Coll Nutr
; 23(6): 763S-7S, 2004 Dec.
Article
en En
| MEDLINE
| ID: mdl-15637231
ABSTRACT
OBJECTIVE:
The importance of chamomile (Chamomilla recutita) is widely known in classical and folk medicine, with the largest group of its effective substances forming the essential oil (chamazulene, alpha-bisabolol, trans-beta-farnesene, spathulenol, cis/trans-en-in-dicycloethers). The increasing need for plant-derived high quality drugs cannot be provided by their collection in the wilderness. METHOD A To preserve the genome of Szabadkigyo. wild type having high (-)-alpha-bisabolol content, we used biotechnological methods.RESULTS:
The roots of organized culture contained beta-eudesmol, which we have identified in the intact roots. Our gas-chromatographic and mass-spectroscopic studies showed that sterile chamomile cultures generated the most important terpenoid and polyin compounds characteristics of the intact plant. We identified berkheyaradulene, geranyl-isovalerate and cedrol, as new components in these cultures. Magnesium (Mg) (370 and 740 mg/l MgSO4) has a positive effect on the growth of organized cultures and also on the quality and quantity of essential oil production. METHOD B Another possible source of variants is available by the genetic transformation of organized cultures by infection with Agrobacterium rhisogenes. With this method, we cultivated chamomile infected by A4-Y clone and investigated the essential oil production by hairy root cultures cultivated on solid and liquid MS B-5 media. The main component of the essential oil of hairy root cultures was trans-beta-farnesene.RESULTS:
We identified alpha-selinene, as a new component in these hairy roots. We studied the growth rate of A4-Y clone on the cited media, containing MgSO4 concentrations 0; 185; 370 and 740 mg/l. The cultures grew most in medium containing 740 mg/l of MgSO4. Essential oil content was compared from hairy root cultures of different Mg containing media and measured by GC and GC-MS methods. Mg has a similar effect on hairy roots as on organized cultures.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aceites Volátiles
/
Manzanilla
/
Magnesio
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Am Coll Nutr
Año:
2004
Tipo del documento:
Article
País de afiliación:
Hungria