Monoclonal antibodies to the mouse p19(Arf) tumor suppressor protein.
Hybrid Hybridomics
; 23(5): 293-300, 2004 Oct.
Article
en En
| MEDLINE
| ID: mdl-15672607
ABSTRACT
The Arf tumor suppressor protein inhibits cell proliferation through both p53-dependent and -independent mechanisms. Two rat monoclonal antibodies raised against a peptide corresponding to amino acids 54-75 of the mouse p19(Arf) protein reacted with the native protein expressed in mammalian cells. Neither antibody detected human or golden hamster Arf proteins. The two antibodies, both of IgG2b isotype, are directed to adjacent epitopes contained within residues 54-62 and 62-75, respectively, of the mouse p19(Arf) protein, and both were highly efficient in detecting p19(Arf) by immunoprecipitation, immunoblotting, and immunofluorescence. One antibody proved useful for immunohistochemical staining of p19(Arf) in fixed sections of mouse testes, revealing low levels of protein expression within the nucleoli of spermatogonia. This indicates that these antibodies should be useful in detecting the endogenous p(19Arf) protein at specific stages of mouse development and during early stages of tumorigenesis.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína p14ARF Supresora de Tumor
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Hybrid Hybridomics
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos