[Further development (MDCK) of live cold-adapted influenza vaccine: cultivation of vaccine strains in production fermenters].
Vopr Virusol
; 50(2): 4-9, 2005.
Article
en Ru
| MEDLINE
| ID: mdl-15881389
ABSTRACT
Optimal conditions were developed for cultivating the cold-adapted reassortant live influenza vaccine (CARLIV) in MDCK cells, which were in their turn cultivated in fermenters with serum-free medium and microcarrier. The use of MDCK cells meets all national and WHO requirements to continuous cells used in the production of biological preparations. CARLIV cultivated under such conditions well preserve their ts-mutations and mutation, which entail substitutions of amino acids, in all CARLIV genome segments. Provided the cultivation conditions are optimal, the output of multivalent CARLIV in a 101 fermenter can reach 100000 doses.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
/
Vacunas contra la Influenza
/
Virus Reordenados
Límite:
Animals
Idioma:
Ru
Revista:
Vopr Virusol
Año:
2005
Tipo del documento:
Article