Application of ES cells for generation of respiration-deficient mice carrying mtDNA with a large-scale deletion.
Biochem Biophys Res Commun
; 333(2): 590-5, 2005 Jul 29.
Article
en En
| MEDLINE
| ID: mdl-15953585
ABSTRACT
In a previous study, we used mouse zygotes as recipients of mtDNA with a large-scale deletion mutation (DeltamtDNA) and generated respiration-deficient mice (mito-mice) carrying DeltamtDNA. In this study, we used mouse ES cells as recipients of DeltamtDNA, and generated mito-mice with DeltamtDNA only when the ES cells carried 17% DeltamtDNA. No chimera mice or their F(1) progenies were obtained from ES cells carrying more than 61% DeltamtDNA. These observations suggest that respiratory defects of ES cells inhibit their normal differentiation into chimera mice and mito-mice, and that ES cells are more effective than zygotes for generation of mito-mice carrying mtDNAs without significant pathogenic mutations.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
ADN Mitocondrial
/
Enfermedades Mitocondriales
/
Riñón
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón