Substrate binding modulates the reduction potential of DNA photolyase.
J Am Chem Soc
; 127(30): 10472-3, 2005 Aug 03.
Article
en En
| MEDLINE
| ID: mdl-16045318
ABSTRACT
The reduction potential of the (FADH-/FADH*) couple in DNA photolyase was measured, and the value was found to be significantly higher than the values estimated in the literature. In the absence of substrate, the enzyme has a reduction potential of 16 +/- 6 mV vs NHE. In the presence of excess substrate the reduction potential increases to 81 +/- 8 mV vs NHE. The increase in reduction potential has physiological relevance since it gives the catalytic state greater resistance to oxidation. This is the first measurement of a reduction potential for this class of DNA-repair enzymes and the larger family of blue-light photoreceptors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Desoxirribodipirimidina Fotoliasa
/
Flavina-Adenina Dinucleótido
Idioma:
En
Revista:
J Am Chem Soc
Año:
2005
Tipo del documento:
Article
País de afiliación:
Estados Unidos