A multivalent HIV-vaccine: development of a plasmid DNA for the expression of HIV envelope glycoproteins with hypervariable V3-loop domains.
Vaccine
; 24(21): 4648-50, 2006 May 22.
Article
en En
| MEDLINE
| ID: mdl-16226356
ABSTRACT
The third hypervariable (V3) loop of the HIV-1 envelope glycoprotein gp120 plays an essential role in the process of viral entry. It contributes to the tropism, coreceptor usage and immune-escape of the virus. We generated a monovalent plasmid DNA and demonstrated the expression of HIV-1 clade B subtype NL4-3 gp120 and gp160 in comparison to a multivalent plasmid DNA encoding for a variety of V3-variants. In contrast to the membrane-anchored gp160, preliminary data demonstrate the monovariant gp120 is expressed in and presented on a human dendritic cell (DC) line, due to a HIVenv-specific re-stimulation of naïve T-cells detected by IFNgamma-ELISPOT assay.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Proteína gp120 de Envoltorio del VIH
/
Proteínas del Envoltorio Viral
/
Vacunas contra el SIDA
/
Vacunas de ADN
Idioma:
En
Revista:
Vaccine
Año:
2006
Tipo del documento:
Article
País de afiliación:
Alemania