Development and validation of a simple reversed-phase high-performance liquid chromatography method for the determination of testosterone in serum of males.
J Pharm Biomed Anal
; 41(2): 527-32, 2006 May 03.
Article
en En
| MEDLINE
| ID: mdl-16406451
ABSTRACT
An isocratic high-performance liquid chromatographic method with detection at 234 nm was developed, optimized and validated for the determination of testosterone in human serum. Propylparaben was used as internal standard. A Hypersil BDS RP-C18 column (150 mmx4.6 mm, 5 microm), was equilibrated with a mobile phase composed of acetonitrile and water (3565, v/v) and having a flow rate of 1 ml/min. The elution time for testosterone and internal standard was approximately 11.6 and 9.9 min, respectively. Calibration curves of testosterone in serum were linear in the concentration range of 1-20 ng/ml. Limits of detection and quantification in serum were 0.4 and 1.1 ng/ml, respectively. Recovery was greater than 92%. Intra- and inter-day relative standard deviation for testosterone in serum was less than 2.1 and 3.9%, respectively. This method was applied to the determination of testosterone serum levels of 12 healthy males and data were correlated with data obtained using a radioimmunoassay method.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testosterona
/
Cromatografía Líquida de Alta Presión
Límite:
Humans
/
Male
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2006
Tipo del documento:
Article
País de afiliación:
Grecia