[Effects of nitric oxide synthases on kidney in early phase of endotoxic shock in macaque].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
; 18(7): 421-4, 2006 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-16831246
ABSTRACT
OBJECTIVE:
To study the pathogenesis of kidney injury in early phase of endotoxic shock.METHODS:
Eleven macaques were anesthetized and randomly divided into two groups an experimental group (n=6), receiving a dose of 2.8 mg/kg lipopolysaccharides (LPS) by intravenous injection, and a control group (n=5), injected with normal saline of 1 ml/kg. The animals were sacrificed 120 minutes following endotoxin injection. With immunohistochemical technique endothelium nitric oxide synthases (eNOS) and inducible nitric oxide synthases (iNOS) protein of the renal tissue were determined. The ultrastructure was studied with electron microscope. Acid phosphatase (ACP) was also assayed.RESULTS:
One hundred and twenty minutes after LPS challenge, damages to the glomerulus and renal tubules were found, and lysosomes were increased in the renal cells. iNOS was positively expressed in vascular endothelium of glomerulus and epithelium of renal tubules in the experimental group, but not in the control group. eNOS was positively expressed in vascular endothelium of glomerulus; and peri-vascular region in both groups.CONCLUSION:
The present findings indicate that NO contributed to the pathogenesis of renal injury in the early phase of endotoxic shock in primates.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Choque Séptico
/
Óxido Nítrico Sintasa de Tipo II
/
Óxido Nítrico Sintasa de Tipo III
/
Riñón
/
Óxido Nítrico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
Asunto de la revista:
MEDICINA DE EMERGENCIA
/
TERAPIA INTENSIVA
Año:
2006
Tipo del documento:
Article
País de afiliación:
China