Evaluation of c-MYC status in primary acquired cholesteatoma by using fluorescence in situ hybridization technique.
Otol Neurotol
; 27(5): 588-91, 2006 Aug.
Article
en En
| MEDLINE
| ID: mdl-16868505
ABSTRACT
OBJECTIVE:
The object of study was to investigate the status of c-MYC oncogene in primary acquired cholesteatoma. STUDYDESIGN:
Descriptive study.METHODS:
Cholesteatoma samples were obtained from 15 patients with primary acquired cholesteatoma during surgical operation. Fluorescence in situ hybridization with a mixed DNA probe, which is specific for c-MYC located on 8q24 and chromosome 8 specific-alpha-satellite DNA probe (dual color), was used on the interphase nuclei.RESULTS:
Copy number of c-MYC oncogene and aneuploidy of chromosome 8 were 21.2% +/- 14.4% and 21.7% +/- 14.8%, respectively. There was no significant difference between copy number of c-MYC and frequency of chromosome 8 aneuploidy (p > 0.05). Ten of 15 cases showed different percentage of c-MYC and chromosome 8 aneuploidy, whereas 5 (33.3%) of 15 cases showed a normal distribution of c-MYC and chromosome 8 signals.CONCLUSION:
The copy number of c-MYC in 10 of 15 cases was found to be high as observed for chromosome 8 aneuploidy in primary acquired cholesteatoma. These findings suggest that the ability of hyperproliferation of primary acquired cholesteatoma might have been related to c-MYC copy number by deregulating c-MYC expression.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Cromosomas Humanos Par 8
/
Genes myc
/
Colesteatoma del Oído Medio
/
Proteínas de Unión al ADN
Límite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Otol Neurotol
Asunto de la revista:
NEUROLOGIA
/
OTORRINOLARINGOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Turquía