A decrease of cell proliferation by hypothermia in the hippocampus of the neonatal rat.
Brain Res
; 1111(1): 36-40, 2006 Sep 21.
Article
en En
| MEDLINE
| ID: mdl-16904084
ABSTRACT
Hypothermia is a potential therapy for cerebral hypoxic ischemic injury of not only adults but also neonates. However, the side effects of hypothermia in the developing brain, where a massive amount of neurogenesis occurs, remain unclear. We investigated the proliferation of neural progenitor cells by systemic application of the thymidine analog 5-bromodeoxyuridine (BrdU) in neonatal rats in a severe hypothermic environment. The rat pups were divided into two groups, a hypothermia group (30 degrees C n=10) and a normothermia group (37 degrees C n=10). After the pups were placed for 21 h in each environment, 100 mg/kg/day of BrdU was injected intraperitoneally to label dividing cells, and then the pups were sacrificed at 24 h. We examined the number of BrdU-labeled cells in the subventricular zone of the periventricle and the subgranular zone of the dentate gyrus. In the hypothermic environment, BrdU-labeled cells significantly decreased in number in the dentate gyrus, but not in the periventricular region. Thus, the severe hypothermic environment induced a decrease of neurogenesis in the neonatal rat. These observations are noteworthy regarding clinical hypothermia therapy following cerebral hypoxic ischemic injury during the perinatal period.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Regulación hacia Abajo
/
Proliferación Celular
/
Hipocampo
/
Hipotermia Inducida
/
Neuronas
Límite:
Animals
/
Humans
/
Newborn
Idioma:
En
Revista:
Brain Res
Año:
2006
Tipo del documento:
Article
País de afiliación:
Japón