An anti-sense c-erbA clone inhibits thyroid hormone-induced expression from the alpha-myosin heavy chain promoter.
J Biol Chem
; 265(11): 6489-93, 1990 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-1690729
ABSTRACT
The effects of thyroid hormone on expression of cardiac myosin heavy chain genes generally are thought to be mediated by nuclear 3,5,3'-triiodo-L-thyronine (T3) receptors that have been identified as the products of the protooncogene, c-erbA. This hypothesis has been tested by transfection of cardiomyocytes in primary culture with a plasmid, pRSVhEACAT-, expressing anti-sense c-erbA mRNA. Because only a low percentage of cells (20%) could be transfected in primary culture an alpha-myosin heavy chain-chloramphenicol acetyltransferase fusion construct was used as a reporter gene. The results indicate that the anti-sense plasmid almost completely blocks T3-induced activity of the reporter gene (less than 1% control) while transfection of a similar amount of the sense construct, pRSVhEACAT+, has no effect. When the c-erbA plasmids were cotransfected with constructs containing T3-independent promoters, no effects on expression were observed. The combined use of an anti-sense construct and a report gene provides a means of studying the role of c-erbA products in intracellular signal transduction even in differentiated, nondividing cells like those of the heart.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Triyodotironina
/
Receptores de Hormona Tiroidea
/
Proto-Oncogenes
/
ARN
/
Regulación de la Expresión Génica
/
Proteínas Proto-Oncogénicas
/
Subfragmentos de Miosina
/
Regiones Promotoras Genéticas
/
Genes
Límite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
J Biol Chem
Año:
1990
Tipo del documento:
Article