Developmental failure of the intra-articular ligaments in mice with absence of growth differentiation factor 5.
Osteoarthritis Cartilage
; 15(4): 468-74, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-17052922
ABSTRACT
OBJECTIVE:
To show the phenotypic characteristics of the knee joints in brachypodism mice (bp mice), which carry a functional null mutation of the growth differentiation factor 5 (GDF5) gene, we investigated the adult and embryonic bp mice.METHOD:
Radiographic and macroscopic examinations of the knee joint of adult bp mice were performed. A histological examination of the knee joint of bp mice from E12.5 to E18.5 was also performed.RESULTS:
Radiographic and macroscopic examinations of the adult bp mice showed anterior dislocation, hypoplastic condyles, and absence of the intra-articular ligaments. Safranin O staining of knee joints of the embryonic bp mice showed severe hypoplasty of the chondroepiphyses and intra-articular ligaments at E16.5. There was no difference in the number and location of 5-bromo-2'-deoxyuridine (BrdU)-positive cells between wild-type and bp mice through E12.5 to E14.5. A terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) study showed excessive cell death of mesenchymal cells of the future knee joint in bp mice at E12.5 and E13.5.CONCLUSION:
bp mice exhibit developmental failure of the condyles and intra-articular ligament of the knee joints.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
6_ODS3_enfermedades_notrasmisibles
Problema de salud:
6_congenital_chromosomal_anomalies
Asunto principal:
Apoptosis
/
Deformidades Congénitas de las Extremidades
/
Proteínas Morfogenéticas Óseas
/
Articulación de la Rodilla
/
Ligamentos Articulares
Límite:
Animals
Idioma:
En
Revista:
Osteoarthritis Cartilage
Asunto de la revista:
ORTOPEDIA
/
REUMATOLOGIA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Japón