Transcriptional profiling of C2C12 myotubes in response to SHIP2 depletion and insulin stimulation.
Genomics
; 89(2): 270-9, 2007 Feb.
Article
en En
| MEDLINE
| ID: mdl-17123777
ABSTRACT
Phosphoinositide lipids generated at the cell membrane are a key component of a variety of signaling pathways. Among several inositol phosphatases that regulate the availability of signaling phosphoinositide lipids, the type II SH2-domain-containing inositol 5-phosphatase (SHIP2; approved gene symbol Inppl1) is believed to have multiple functions, including the regulation of insulin signaling and cytoskeletal functions. To understand the function of SHIP2 in C2C12 muscle cells, we depleted SHIP2 through the use of RNA interference and analyzed the global effect of SHIP2 depletion on gene expression using Affymetrix microarrays containing approximately 45,000 mouse probe sets. Expression of SHIP2-targeting small-hairpin RNA in differentiated C2C12 muscle cells led to >80% decrease in SHIP2 mRNA and 60-80% decrease in SHIP2 protein, which resulted in significant gene expression changes linked to cytoskeletal functions, including altered expression of adducin-alpha, pallidin, stathmin-like-2, and synaptojanin-2 binding protein. Insulin treatment of C2C12 muscle cells caused transcriptional changes associated with known signaling pathways. However, SHIP2 depletion had no discernible effect on insulin-regulated gene expression. Taken together, our results suggest that SHIP2 is involved in the regulation of cytoskeletal functions, but a large reduction of SHIP2 in C2C12 muscle cells is not sufficient to affect insulin-mediated gene expression.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Monoéster Fosfórico Hidrolasas
/
Fibras Musculares Esqueléticas
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
Genomics
Asunto de la revista:
GENETICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos