Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads.
Hum Reprod
; 22(4): 1052-9, 2007 Apr.
Article
en En
| MEDLINE
| ID: mdl-17208942
ABSTRACT
BACKGROUND:
Cellular and molecular mechanisms leading to elongated sperm heads are not known. We have analysed the nuclear status of spermatozoa with elongated heads.METHODS:
Fourteen men with at least 30% of spermatozoa with an elongated nucleus were studied and compared with five fertile men as controls. Sperm morphology was analysed by a quantitative ultrastructural analysis. Sperm chromosomal content was assessed by three-colour fluorescence in-situ hybridization (chromosomes X, Y, 18). Y chromosome microdeletion and karyotype were analysed.RESULTS:
Elongated sperm head rates of the patients were 46.9% (30-75 versus 0-2% in the control group) by light microscopy and 34.4% by electron microscopy. In all patients, the chromatin was poorly condensed in elongated sperm heads (50% of elongated nuclei). No anomalies of sperm biochemical markers were found. All the men showed normal karyotype (46,XY) and absence of Y chromosome microdeletion. Aneuploidy rates of gonosomes and chromosome 18 were significantly increased in patients (1.64- and 3.6-fold, P = 0.006 and 0.026, respectively).CONCLUSIONS:
This study demonstrates that impaired chromatin compaction and slightly increased chromosome aneuploidies are found in spermatozoa with an elongated head, suggesting possible mechanisms such as meiotic non-disjunctions or spermiogenesis anomalies.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Núcleo Celular
/
Cromosomas
/
Infertilidad Masculina
/
Aneuploidia
Límite:
Humans
/
Male
Idioma:
En
Revista:
Hum Reprod
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Francia