Coronin 3 involvement in F-actin-dependent processes at the cell cortex.
Exp Cell Res
; 313(5): 878-95, 2007 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-17274980
ABSTRACT
The actin interaction of coronin 3 has been mainly documented by in vitro experiments. Here, we discuss coronin 3 properties in the light of new structural information and focus on assays that reflect in vivo roles of coronin 3 and its impact on F-actin-associated functions. Using GFP-tagged coronin 3 fusion proteins and RNAi silencing we show that coronin 3 has roles in wound healing, protrusion formation, cell proliferation, cytokinesis, endocytosis, axonal growth, and secretion. During formation of cell protrusions actin accumulation precedes the focal enrichment of coronin 3 suggesting a role for coronin 3 in events that follow the initial F-actin assembly. Moreover, we show that coronin 3 similar to other coronins interacts with the Arp2/3-complex and cofilin indicating that this family in general is involved in regulating Arp2/3-mediated events.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Actinas
/
Proteínas de Microfilamentos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Exp Cell Res
Año:
2007
Tipo del documento:
Article
País de afiliación:
Alemania