[Cloning and sequence analysis of a partial gene of Trichomonas vaginalis dsRNA virus].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 24(5): 389-90, 2006 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-17361826
ABSTRACT
A pair of degenerate primers were designed following the published nucleotide sequence of Trichomonas vaginalis virus(U08999, NC003873, NC003824, NC003834). Using the total nucleic acids extracted from the Trichomonas vaginalis as template, RT-PCR was performed with the primers to obtain a fragment of the TVV. The product was cloned, sequenced and compared with the sequences available in the GenBank. The size of the amplified gene was 1 454bp, which shares 82.9% sequence identity with the Trichomonas vaginalis virus T1.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus ARN
/
Trichomonas vaginalis
/
ARN Bicatenario
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
Asunto de la revista:
PARASITOLOGIA
Año:
2006
Tipo del documento:
Article
País de afiliación:
China