On-line determination of serum bactericidal activity using recombinant luminescent bacteria.
Bull Exp Biol Med
; 142(2): 234-8, 2006 Aug.
Article
en En, Ru
| MEDLINE
| ID: mdl-17369948
ABSTRACT
Intensity of luminescence quenching in recombinant strains of Escherichia coli with cloned lux-operones by human blood serum is directly proportional to the degree of bactericidal effect assessed by nephelometric and bacteriological methods. This correlation was most characteristic of E. coli with luminescence genes from Photobacterium leiognathi, which substantiates its use in the development of the kinetic bioluminescent method to determine of serum bactericidal activity. The possibility of using this method for evaluation of activity of classic and alternative pathways of compliment activation was demonstrated by using zymosan or EGTA-Mg(2+)-treated sera and C1-C5-deficient sera.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Actividad Bactericida de la Sangre
/
Activación de Complemento
/
Escherichia coli
Límite:
Humans
Idioma:
En
/
Ru
Revista:
Bull Exp Biol Med
Año:
2006
Tipo del documento:
Article