[Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene].

ABSTRACT

OBJECTIVE: To report the use of Dicer to cleave double-stranded RNA (dsRNAs) into small interference RNAs (D-siRNAs) that can target multiple sites within an mRNA, and to acquire an new method to cure inflammation of the airway and tumor. METHODS: Using RiboMAX Large Scale RNA Production Systems-SP6 and T7 kit were used to transcribe A549 cell COX-2 DNA into RNA (dsRNAs). We mixed dsRNAs with Dicer in the reaction buffer. We recovered siRNAs using RNA Purification Column. RESULTS: Dicer efficiently converted double-stranded RNA of COX-2 into small interference RNAs of 21 approximately 23 bp. CONCLUSION: Dicer efficiently converts double-stranded RNA (dsRNA) into small interference RNAs (D-siRNAs of 21 approximately 23 bp).