Expression of feline angiotensin converting enzyme 2 and its interaction with SARS-CoV S1 protein.
Res Vet Sci
; 84(3): 494-6, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-17658563
ABSTRACT
Feline angiotensin converting enzyme 2 (fACE2) gene was amplified from domestic cat lung with RT-PCR, cloned and sequenced. The complete coding region is 2418bp in length and is the closest to human ACE2 among known ACE2 homologs of non-primate animals. The N terminal fragment 19- 367 aa was expressed in Escherishia coli. Both Western blotting and ELISA demonstrated that fACE2 could react with SARS-CoV S1 protein as efficiently as ACE2 of Vero E6 cells did.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de la Matriz Viral
/
Peptidil-Dipeptidasa A
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Res Vet Sci
Año:
2008
Tipo del documento:
Article
País de afiliación:
China