Identification of a 150 bp cis-acting element of the AtNRT2.1 promoter involved in the regulation of gene expression by the N and C status of the plant.
Plant Cell Environ
; 30(11): 1366-80, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-17897408
ABSTRACT
The Arabidopsis thaliana AtNRT2.1 gene, which encodes a NO(3)(-) transporter involved in high-affinity uptake by the roots, is a molecular target of several mechanisms responsible for the regulation of root NO(3)(-) acquisition by the N status of the plant. All levels of AtNRT2.1 expression (promoter activity, transcript level, protein accumulation, transport activity) are coordinately up-regulated in the presence of NO(3)(-), and repressed by downstream N metabolites. Transgenic plants expressing the GUS reporter gene under the control of upstream sequences of AtNRT2.1 have been studied to identify elements targeted by these two regulatory mechanisms. A 150 bp sequence located upstream of the TATA box that is required for both stimulation by NO(3)(-) and repression by N metabolites of the promoter has been identified. This sequence is able to confer these two regulations to a minimal promoter. Split-root experiments indicate that the stimulation of the chimaeric promoter by NO(3)(-) occurs only at the local level, whereas its repression by N metabolites is mediated by a systemic signal spread to the whole plant. The activity of the cis-acting 150 bp element is also regulated by sucrose supply to the roots, suggesting a possible interaction between N and C signalling within this short region. Accordingly, multiple motifs potentially involved in regulations by N and/or C status are identified within this sequence by bioinformatic approaches. This is the first report of such a cis-acting element in higher plants.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carbono
/
Regiones Promotoras Genéticas
/
Arabidopsis
/
Regulación de la Expresión Génica de las Plantas
/
Proteínas de Transporte de Anión
/
Proteínas de Arabidopsis
/
Nitrógeno
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Plant Cell Environ
Asunto de la revista:
BOTANICA
Año:
2007
Tipo del documento:
Article